New Johns Hopkins Study Identifies Pervasive RT-qPCR Artifact in CRISPR Knockdown Studies and How UltraMarathonRT® Solves It

Summary

Johns Hopkins University study identifies an RT-qPCR artifact in CRISPR knockdown studies where guide RNAs suppress reverse transcription accuracy. UltraMarathonRT, utilizing strand-displacing reverse transcriptase, is presented as a solution to restore accurate knockdown quantification.